Transferrin (human) AlphaLISA Detection Kit, 5,000 Assay Points

Formats:

• Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Human Transferrin is a 679 amino acid protein (80 kDa) composed of alpha helices and beta sheets that form two distinct domains that each contain an iron binding site. This protein functions to control the level of free iron found in biological fluids. The liver is the main site of transferrin synthesis and its main role is to deliver iron to all tissues. Normal human serum transferrin levels range from 200-360 mg/dL. The transferrin protein has been implicated in several disease states including iron deficiency anemia (high serum transferrin levels) and liver toxicity. The present kit permits detection of human transferrin (i.e. analyte) in different sample matrices, including DMEM cell culture media.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.