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Thymidine, [Methyl-3H]-, Specific Activity: 6.7 Ci/mmol, 250µCi

Thymidine, tritiated on the methyl group. This product is supplied steri-packaged in an aqueous solution, and is typically used in DNA cell proliferation assays.

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NET027250UC
250 µCi
269.00 EUR
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NET027001MC
1 mCi
491.00 EUR
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NET027005MC
5 mCi
1185.00 EUR
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Detail Information

250 µCi quantities of [Methyl-3H]-Thymidine are available for your research. Application of [3H]Thymidine can be found in: carboxy-terminal PTH fragments stimulating incorporation in vascular endothelial cells, quantification of granulocyte-macrophage colony-stimulating factor (GM-CSF) hypersensitivity in juvenile myelomonocytic leukemia (JMML), influence of parasympathetic non-adrenergic, non-cholinergic mechanisms on uptake (rat) in response to mastication, measurement of dendritic cell induced T cell response by intracellular ATP levels and uptake in human immunology, etc. Special Information: If aqueous solutions of thymidine are stored frozen (-20°C), care should be taken to protect the material from U.V. light during the thawing process to prevent possible dimerization. When [methyl-3H]thymidine is incorporated into cellular DNA and undergoes radiolytic decomposition, [3H]5-hydroxymethyl-2''-deoxyuridine (HMdU) is formed.* The formation of HMdU through transmutation of [methyl-3H]thymidine occurs at the rate of B-decay, which is 0.017% per day. The transmutation product is not volatile. *References S. Apelgot and B. Ekert, J. Chem. Phys. 60, 505 (1963). K. Fink, R. E. Cline, and R. M. Fink, Anal. Chem., 35, 389 (1963). Specifications and Criteria for Biochemical Compounds, Third Edition (ISBN 0-309-01917-6), National Academy of Sciences - National Research Council, Washington, D.C. 1972. G. W. Teebor, K. Frenkel, and M. S. Goldstein, Proc. Natl. Acad. Sci. USA 81, 318 (1984). H. Tono, S.S. Cohen, J. Biol. Chem. 237, 1271 (1962). M. Nemer, J. Biol. Chem. 237, 143 (1962).

Specifications

Detection Method Radiometric
Label Position Specifically Labeled
Molecular Weight 242.2
Product Brand Name NEN Radiochemicals
Radioisotope H-3
Radionuclide 3H
Shipping Condition Ambient
Special Ordering Information This is a radioactive product - shipping address must have a license to receive radioactive materials.
Unit Size 250 µCi
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Radiometric Reagents Guide 2010-2011

For over 50 years PerkinElmer has been a leading supplier of radiochemicals, liquid scintillation cocktails, vials and nuclear counting detection instruments. Today is no different. We have always been committed to providing you products for all of your radiometric needs and we are still committed today.

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