Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling.
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LANCE® Eu-W8044-DTA Chelate is optimized for the labeling of proteins and peptides containing at least one primary aliphatic amino group. The labeled compound can be used in LANCE® homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assays and fluorescence quenching assays (TF-FQA).
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC)]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE|
|Shipping Condition||Blue Ice|
|Unit Size||1 mg|
|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 82 KB|
|Guide||LANCE Protein: Protein Interaction Quick Start Guide||PDF 1 MB|
|Guide||LANCE TR-FRET Immunoassay Conversion Guide||PDF 3 MB|
|Guide||LANCE TR-FRET protein-protein interaction assay development guide||PDF 1 MB|
|Application Note||Biochemical Binding ADCC Assays Utilizing LANCE Toolbox Reagents for the Characterization of hIgGs a||PDF 1 MB|