Our Membrane Target System membranes are prepared from cells that express recombinant or endogenous receptors. Selected for optimal performance in binding assays.
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Membrane Target Systems are quality assured frozen membranes from cells that express recombinant or endogenous receptors. We submit every batch of receptor to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPgS data is also provided for some of our Gi coupled receptors.
Membranes are carefully prepared and ready for a variety of HTS applications, including radioligand binding (using either proximity methods, such as FlashPlate, or classical filtration methods). Products are packaged as frozen crude membrane preparations. One assay unit is defined as micrograms of protein, defined by competition binding assay (filtration). A complete product description and recommended protocol are included on the Certificate of Analysis.
Some of our receptors may be restricted for sale in specified countries. Please inquire.
|Assay Validated||Binding, GTPγS|
|Second Messenger Release||GTP exchange|
|Shipping Condition||Dry Ice|
|Unit Size||400 units|