Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits. The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).
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Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits.
This kit is designed to be used with any other AlphaLISA SureFire Ultra assay kit to allow multiplexed measurement of pERK with the target of another AlphaLISA SureFire Ultra kit (“Target 2”). The “Alpha 615 anti-pERK (mIgG1) Acceptor Beads” in this kit are directly-conjugated with an antibody to the phosphorylated site on p-ERK and, in combination with the biotinylated antibody provided in this kit, will generate a 615 nm signal relative to the levels of p-ERK. The “Alpha 545 CaptSure™ Acceptor Beads” are coated with the CaptSure™ agent, which binds CaptSure-tagged antibodies, and are used in place of the “AlphaLISA® CaptSure™ Acceptor Beads” from the AlphaLISA SureFire Ultra kit. The use of the CaptSure beads from this kit will thus be shifting the wavelength at which the Target 2 will be read (i.e. Target 2, from the AlphaLISA SureFire Ultra kit, will now be read at 545 nm instead of the initial 615 nm in the AlphaLISA SureFire Ultra format).
|Assay Target Class||Phosphoprotein|
|Experimental Type||In vitro|
|Product Brand Name||Alpha SureFire Ultra Multiplex, AlphaPlex|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 124 KB|
|Application Note||Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra||PDF 2 MB|
|Application Note||Simultaneous Detection of Total and Phosphorylated Protein using Alpha SureFire Ultra Multiplex||PDF 1 MB|
|Manual||Protocol for Alpha SureFire Ultra Multiplex HV pERK Single Kit||PDF 643 KB|
|Technical Note||Multiplex Assays with Alpha SureFire Ultra Multiplex Technology using the EnVision Multilable Plate||PDF 2 MB|
|Poster||Rapid homogeneous detection systems for cellular signaling pathways||PDF 805 KB|
|Flyer||Alpha SureFire Technology||PDF 720 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)|