PerkinElmer
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Purinergic P2Y11 AequoScreen Cell Line

Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.

Part Number ES-293-A
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This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

Detail Information

AequoScreen® Double Transfected Cell Line: Human recombinant P2Y11 receptor in aequorin 1321N1 host cell. We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant receptor in a functional assay. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

Features:

  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z', CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput
  • Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

Specifications

Assay Target Class GPCR
Assay Target Type Cell line
Assay Validated Calcium Luminescence
G-Alpha Natural Receptor Gq/G11
Host Cell 1321N1
Product Brand Name AequoScreen
Receptor Type Purinergic P2Y
Second Messenger Release Calcium flux
Shipping Condition Dry Ice
Therapeutic Area Inflammation, Metabolic
Unit Size 5 million cells
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Application Note

AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines

Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.

PDF 607 KB
Aequorin bioluminescence assays on MicroBeta2 LumiJET for studying Ca2+-coupled GPCRs and ion channels

Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme.

PDF 152 KB

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Aequorin FLIPR

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