Opal™ is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed. The Opal 4-Color Automation Immunohistochemistry Kit is designed for use on the Leica Biosystems BOND RX, and is configured and validated for imaging on the Vectra® and Mantra™ systems. This kit contains reagents to stain 50-slides at the recommended dilution ratio.
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The Opal 4-Color Automation IHC Kit contains:
Users should source primary antibodies and other workflow reagents separately. The Opal 4-Color Automation IHC Kit was optimized for use on the Leica Biosystems BOND RX and configured and validated for imaging on the Vectra® and Mantra™ systems. Kits contain enough reagents to stain 50-slides at the recommended 1:150 dilution ratio.
Opal will enable you to:
Automating Opal staining brings the benefit of:
|Detection Method||MSI, Fluorescence|
|Fluorophore||Opal 520, Opal 570 and Opal 690|
|Product Brand Name||Opal|
|Quantity in a Package Amount||1.0 per pack|
|Shipping Condition||Blue Ice|
|Unit Size||4-color kit|
This ready-to-use amplification diluent has been optimized and specifically formulated for Opal multiplex IHC staining on the Leica BOND RX.