PerkinElmer
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AlphaScreen FLAG (M2) Detection Kit, 50,000 Assay Points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FLAG conjugated AlphaScreen Acceptor beads. 

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For research use only. Not for use in diagnostic procedures.

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Unit Size
List Price
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6760613C
500 assay points
187.00 EUR
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6760613M
10,000 assay points
2986.00 EUR
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6760613R
50,000 assay points
14300.00 EUR
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Detail Information

This kit contains enough reagents to run 50,000 wells in 384-well format, using a 25 µL reaction volume. The M2 antibody clone can recognize N-terminal, C-terminal, and internal FLAG tags.

This bead kit can be used for many applications, including:

 

  • Protein-protein interaction assays
  • Protein-peptide interaction assays
  • FLAG-tagged protein detection and quantitation
  • Protein expression
  • Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is FLAG-tagged, and associates with the AlphaScreen anti-FLAG Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated FLAG probe provided in the kit.

Specifications

Antibody Conjugates Streptavidin/Anti-FLAG
Automation Compatible Yes
Product Brand Name AlphaScreen
Shipping Condition Blue Ice
Unit Size 50,000 assay points