The LANCE® Ultra Mouse IL-1β Detection Kit is designed for detection and quantitation of mouse interleukin 1β in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF1503C||500 Assay Points||826.00 EUR|
|TRF1503M||10,000 Assay Points||11000.00 EUR|
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The LANCE Ultra Mouse IL-1β Detection Kit is designed for detection and quantitation of mouse interleukin 1β in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
The mouse Interleukin 1 beta (IL1ß) is produced as a 269 amino acid precursor that matures by proteolysis to its 152 amino acid active form. In humans, IL1α and IL1ß are central players of the immune response, displaying roles in inflammation both at local and systemic levels. IL1ß is functionally equivalent to IL1α. Its production has been reported in many cell types including brain cells, as well as monocytic and peripheral blood mononuclear cells. Among the biological activities of IL1 is the stimulation of T-helper cells, which then secrete IL2 and express IL2 receptor. IL1 acts directly on B-cells, promoting their proliferation and the synthesis of immunoglobulins. It supports tumor cytotoxicity mediated by monocytes and induces tumor regression. It has been shown that IL1 also promotes wound healing.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
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|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 77 KB|
|Application Note||High Throughput Quantitation of Cytokine Biomarkers using LANCE Ultra TR-FRET Assays||PDF 1 MB|
|Application Note||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|
|Technical Note||Comparison of LANCE Ultra and HTRF Assays for Measuring the Presence of Pro-inflammatory Cytokines||PDF 277 KB|
|Data Sheet||Tech data sheet: LANCE mouse IL1ß TRF1503||PDF 596 KB|
|Flyer||LANCE Ultra Biomarker Detection Kits||PDF 585 KB|