PerkinElmer
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IgA (human) LANCE Ultra TR-FRET Detection Kit, 500 assay points

The LANCE® Ultra Human IgA Detection Kit is designed for detection and quantitation of human immunoglobulin A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF1262C
500 Assay Points
826.00 EUR
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TRF1262M
10,000 Assay Points
11000.00 EUR
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Detail Information

The LANCE Ultra Human IgA Detection Kit is designed for detection and quantitation of human immunoglobulin A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Immunoglobulin A (IgA) is a class of antibody mainly present in mucosal areas. IgA is essentially secreted in a dimeric form, which is maintained in place by the J-chain, a polypeptide of 15 kDa rich in cysteine residues. There are two isotypes of IgA: IgA1 is prevalent in the serum while IgA2 is prevalent in secretions. IgA is present in the gut, respiratory tract, urogenital tract, and in mucous secretions like saliva, tears, colostrum, vaginal fluid, and breast milk. IgA is relatively resistant to degradation and can remain active in extreme environments such as the digestive tract. IgA binds to pathogen, but is a poor activator of the complement. IgA deposition in the glomerulus is associated with IgA nephropathy.

Specifications

Assay Target IgA
Assay Target Class Antibody
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Target Species Human
Therapeutic Area Biologics/Bioprocess
Unit Size 500 Assay Points