PerkinElmer
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Transferrin (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human Transferrin Detection Kit is designed for detection and quantitation of human transferrin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF1311C
500 Assay Points
826.00 EUR
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TRF1311M
10,000 Assay Points
11000.00 EUR
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Detail Information

The LANCE® Ultra Human Transferrin Detection Kit is designed for detection and quantitation of human transferrin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Human Transferrin is a 679 amino acid protein (80 kDa) composed of alpha helices and beta sheets that form two distinct domains that each contain an iron binding site. This protein functions to control the level of free iron found in biological fluids. The liver is the main site of transferrin synthesis and its main role is to deliver iron to all tissues. Normal human serum transferrin levels range from 200-360 mg/dL. The transferrin protein has been implicated in several disease states including iron deficiency anemia (high serum transferrin levels) and liver toxicity. The present kit permits detection of human transferrin (i.e. analyte) in different sample matrices, including DMEM cell culture media.

Specifications

Assay Target Transferrin
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 500 Assay Points