PerkinElmer
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IgG (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human IgG Detection Kit is designed for detection and quantitation of human IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF1205C
500 Assay Points
826.00 EUR
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TRF1205M
10,000 Assay Points
11000.00 EUR
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Detail Information

The LANCE® Ultra Human IgG Detection Kit is designed for detection and quantitation of human IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

The glycoprotein immunoglobulin G (IgG), a major effector molecule of the humoral immune response in human, accounts for about 75% of the total immunoglobulins in plasma of healthy individuals whereas IgM, IgA, IgD and IgE, each of which has characteristic properties and functions, constitute the remaining 25%. The basic IgG molecule has a four-chain structure, comprising two identical heavy (H) chains and two identical light (L) chains, linked together by interchain disulfide bonds. Four IgG subclasses have been identified showing their most conspicuous differences in the amino acid composition and structure of the 'hinge region'. The present kit recognizes total IgGs Fc fragment.

 

Specifications

Assay Target IgG
Assay Target Class Antibody
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Biologics/Bioprocess
Unit Size 10,000 Assay Points