PerkinElmer
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Glucagon (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human Glucagon Detection Kit is designed for detection and quantitation of human glucagon in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF1312C
500 Assay Points
826.00 EUR
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TRF1312M
10,000 Assay Points
11000.00 EUR
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Detail Information

Attention: Glucagon Analyte included in this kit is provided in solution. Please store at -20°C upon receipt of the kit. Upon the first use of the kit aliquot the analyte and store the aliquots at -20°C to avoid further freeze/thaw cycles.

The LANCE® Ultra Human Glucagon Detection Kit is designed for detection and quantitation of human glucagon in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Glucagon is a 29 amino acid peptide hormone produced by the pancreas. Glucagon generally functions as a counterregulatory homrone opposing the actions of insulin to maintain appropriate levels of blood glucose. Normal human serum glucagon levels range from 50-200 pg/mL. The glucagon:insulin ratio controls the rate of gluconeogenesis and glycogenolysis, disruption of this ratio can have severe metabolic implications. The present kit permits detection of glucagon (i.e. analyte) in different sample matrices.

Specifications

Assay Target Glucagon
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 500 Assay Points