LANCE Ultra phospho-eIF2A (Ser51) kits are designed for the detection of eIF2A (phosphorylated at S51) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF4008C||500 Assay Points||1275.00 EUR|
|TRF4008M||10,000 Assay Points||5700.00 EUR|
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Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4008S.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
Eukaryotic initiation factor 2 (eIF2) is a heterotrimer which is involved in the initiation of translation in eukaryotes. EIF2 binds to two other partners, GTP and Met-tRNAi, and forms the 43S pre-initiation complex upon binding the 40S subunit. Under conditions of cellular stress, the alpha subunit can be phosphorylated at S51 to downregulate protein translation.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 77 KB|
|Guide||LANCE Ultra TR-FRET Cellular Phosphorylation Quick Start Guide||PDF 1 MB|
|Application Note||LANCE Ultra TR-FRET-based Detection and Modulation of Phosphorylated STAT3 Levels in Human Cells||PDF 2 MB|
|Manual||Protocol for LANCE Ultra TR-FRET phospho eIF2a cellular detection assay||PDF 475 KB|
|Technical Note||Development of LANCE TR-FRET Kits for Cellular Phosphoprotein Detection||PDF 1 MB|