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LANCE Ultra KinaSelect™ Ser/Thr Kit, 5 X 250 assay points

LANCE® Ultra TR-FRET Ser/Thr kinase substrate selection kit, containing five different ULight-peptide substrates and corresponding Europium anti-phospho antibodies for kinase reactions.

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TRF0300-C
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Detail Information

The LANCE® Ultra KinaSelect™ Ser/Thr kit is
intended for selecting the optimal peptide substrate for serine and
threonine (Ser/Thr) kinases. Kinase activity is measured in a
LANCE® time-resolved fluorescence resonance energy transfer
(TR-FRET) assay using five different ULight™-labeled
peptide substrates with their corresponding europium (Eu)-labeled anti-phospho-antibodies. Substrate/antibody pairs giving the best
performance can then be used for further assay development and
optimization. The five ULight™-labeled peptides and
Eu-labeled antibodies selected for the KinaSelect kit were found to
generate signal with over 80% of a panel of 184 Ser/Thr kinases.


LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:


  • Test conditions: Rapid assay setup to move straight into optimized assay performance

  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing

  • Phospho-motifs: Substrate sequences are provided

  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.


Specifications

Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Unit Size 1 Kit
Resources, Events & More
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Application Note

Comparison of LANCE Ultra TR-FRET to PerkinElmer's Classical LANCE TR-FRET Platform for Kinase Applications

Protein kinases play a major role in normal cellular functions such as cell proliferation, angiogenesis, and cell adhesion. Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. This makes kinases crucial targets for the discovery and development of new drugs.

PDF 805 KB
Development and Optimization of Kinase Assays using New LANCE Ultra TR-FRET Reagents

Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. Because of their function, kinases are crucial targets for drug discovery. Analysis of the human genomehas revealed the existence ofnearly 520 genes encoding kinases.

PDF 583 KB

Brochure

Guide

LANCE Ultra Substrate Selection Guide

LANCE® Ultra TR-FRET reagents comprise the widest portfolio of validated kinase assay offerings available for rapid, sensitive and robust screening of purified kinase targets in a biochemical format.

PDF 1 MB

Manual

LANCE Ultra Kinaselect Ser/Thr Kit

LANCE Ultra TR-FRET assays use the proprietary W1024 europium chelate (Eu) donor dye with ULight, a low molecular weight acceptor dye with a red-shifted fluorescent emission.

PDF 112 KB

Poster

Comparison of LANCE Ultra with Other TR-FRET Platforms

Protein kinases play a major role in normal cellular functions such as cell proliferation, angiogenesis, and cell adhesion. Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. This makes kinases crucial targets for drug discovery. Analysis of the human genome has revealed the existence of nearly 520 genes encoding kinases.

PDF 356 KB
Development, Automation and Miniaturization of High-Throughput Serine/Threonine Kinase Assays Using the LANCE® Ultra Platform

Protein kinases are involved in the regulation of many aspects of the cellcycle, including proliferation, differentiation, secretion and apoptosis. Aberrant protein kinase expression or functioning is a cause orconsequence of many human diseases. As a result, kinases have become extremely popular for drug discovery programs and this has prompted the development of many kinase assay technologies suitable for highthrough putscreening (HTS).

PDF 364 KB
Novel Substrates for Fluorescence-based Protein Tyrosine Kinase Assays

Protein tyrosine kinases (PTK) are involved in the regulation of many aspects of the cell cycle, including proliferation, differentiation, secretion and apoptosis. Aberrant protein kinase expression or function is a cause or consequence of many human diseases like cancer and atherosclerosis. As a result, kinases became very important targets in drug discovery.

PDF 276 KB
Poster LANCE Ultra Tyrosine kinases

Of the 518 known human kinases, 90 are tyrosine (Tyr) kinases. Protein Tyr kinases play a key role in signal transduction and normal cell growth. They are also involved in numerous proliferative diseases like cancer and atherosclerosis, in addition to a number of autoimmune diseases. For these reasons, Tyr kinases are often targets for new drug discovery in many laboratories involved in basic R&D, disease and therapeutics research, and HTS.

PDF 276 KB