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Harmony High Content Imaging and Analysis Software

Only the powerfully simple Harmony® High-Content Imaging and Analysis Software unleashes the full potential of your Operetta®, Opera Phenix or Operetta CLS system.

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Harmony provides a complete solution with all the tools you need to set up assays and automate your high-content imaging experiments, acquire images and analyze data and then store, retrieve and present the results.

Designed for biologists, Harmony’s workflow-based interface makes the whole process of high-content analysis straightforward, even for new users with little microscopy or programming knowledge.

With regular upgrades this efficient, flexible solution for gathering unbiased data from your cell samples, keeps ahead of your increasing research needs.

The powerful way to unbiased, statistically relevant data

  • Fully automated solution that takes the human bias out of cellular image analysis
  • Workflow based interface makes it quick and easy for biologists to turn their biology into quantitative data
  • Intuitive plate wizard for simple set-up of new plate types – automatically detects the important parameters plate bottom height and plate bottom thickness
  • Range of ready-made solutions so that you can get productive with high-content image analysis right away using the pre-configured templates for common applications
  • User-configurable image analysis building blocks for creating high-content image analysis applications tailored to fit your research
  • Analysis capabilities for a wide range of applications, from basic research to assay development and screening
  • Texture analysis for applications where intensity and morphology are not suitable or do not deliver robust readout and reproducible results
  • STAR analysis provides a more detailed analysis of cellular morphology to accurately differentiate cellular phenotypes with parameters that describe cellular symmetry and compactness as well as the intracellular distribution of fluorescence
  • Cell tracking tools for analyzing temporal changes of biological systems and cell dynamics in 2D
  • Advanced building blocks for easier analysis of complex applications such as FRET
  • XYZ View shows an xy-, xz- and yz-section through an image stack of a thick sample e.g. microtissue
  • NEW: Global Image – creates stitched images from multiple fields of view
  • NEW: Multiscale analysis – regions found in the “global image” can be used for analysis in the original images, e.g. a stem cell colony that spans two fields of view can be segmented in the global image and cell phenotyping can be done in the high resolution field of view
  • NEW: Windows 10 support

Access, store, share. Manage your results efficiently

  • Experiment metadata, such as dyes / filters, objectives, plate type, fields, z-stack or exposure, are stored in the database for future reference
  • Sortable database for quick and easy access to images, metadata and results
  • View and analyze data from any computer that has an installation of the Harmony software, freeing up the Operetta, Operetta CLS or Opera Phenix for others to use

Creating algorithms has never been this easy

PhenoLOGIC enables biologists to train software to develop the image analysis algorithms. While other systems may require an image analysis expert to create an algorithm, PhenoLOGIC uses proprietary machine-learning technology to make it easy for you to do it on your own. Using a learn-by-example approach, images can be segmented with just a few clicks of the mouse and then tailored algorithms developed quickly and easily.

More accurately locate your object of interest for greater efficiency

Harness the power of intelligent image acquisition with PreciScan for more efficient high-content imaging and analysis. This optional plug-in for the Harmony software enables you to more accurately target your object of interest for significantly reduced acquisition and analysis times, particularly valuable for 3D microtissue and rare event studies.

Assay Groups

Whichever assay you are working on, Harmony software offers a solution:

  • Apoptosis
  • Cell Cycle
  • Cell Differentiation
  • Cell Migration
  • Cell Proliferation
  • Cell Shape Changes
  • Cytoskeletal Rearrangement
  • Cytotoxicity
  • Fluorescence in Situ Hybridization (FISH)
  • Lipid Droplet Analysis
  • Neurite Outgrowth
  • Protein Expression
  • Receptor Activation
  • RNAi Screening
  • Signaling Pathway Analysis
  • Transcription Factors

Resources, Events & More

All (32)
Resource Type File Name File Format
Brochure 3D Cell Culture Solutions Brochure PDF  4 MB
Application Note 3D Analysis of Cell Invasion using Operetta PDF  1 MB
Application Note A Multiparametric Live-Cell Cytotoxicity Analysis using the Operetta PDF  1 MB
Application Note Analysis of Stem Cell Differentiation using Operetta PDF  1 MB
Application Note Automated Single Cell Tracking using Operetta PDF  2 MB
Application Note Comparison of HCA ImagAmp and Conventional Indirect Immunofluorescence for High-Content Analysis Applications PDF  508 KB
Application Note Cytotoxicity Studies on 3D Primary Liver Microtissues PDF  2 MB
Application Note Cytotoxicity Studies on Live Primary Human Hepatocytes using Operetta PDF  2 MB
Application Note Determination of Cell Cycle Phase using the Operetta PDF  618 KB
Application Note Image-based Quantification of PKC Activation using Operetta PDF  827 KB
Application Note Image-based Quantification of the Huntingtin Protein using the Operetta PDF  833 KB
Application Note Imaging Bile Canaliculi in 3D Liver Microtissues using the Opera Phenix HCS System PDF  2 MB
Application Note Measuring FRET using the Opera Phenix High-Content Screening System PDF  1 MB
Application Note Quantitative Analysis of 3D Microtissue Growth and Biomarker Intensity PDF  916 KB
Application Note Quantitative Analysis of Embryoid Bodies Using the Operetta System PDF  921 KB
Application Note Screening Epigenetic HDAC Inhibitors – A Workflow Comprising HTS and HCS PDF  1 MB
Application Note Signal Amplification Strategies for Rapid and Sensitive High Content Analysis PDF  776 KB
Application Note Studying Kidney Injury using Operetta PDF  1 MB
Application Note Studying NfkB signaling dynamics using Operetta PDF  864 KB
Application Note Visualization of Cathepsin Activity using Operetta and ProSense 680 PDF  1 MB
Technical Note Improved 3D Imaging with Opera Phenix HCS System PDF  1 MB
Technical Note Workflows for In-depth High Content Analysis PDF  1 MB
Poster Automation of both sample preparation and analysis of a cell migration assay PDF  911 KB
Poster FRET-based Quantification of Protein-Protein Interactions using the Opera Phenix HCS System PDF  808 KB
Poster Image-based quantification of HCS cell-based assays using the Operetta/Harmony imaging platform PDF  867 KB
Poster Pushing the Boundaries of 3D Microtissue Analysis using High Content Imaging PDF  990 KB
Poster Screening HDAC Inhibitors – A Workflow Comprising High Throughput And High Content Screening PDF  720 KB
Poster Studying kidney injury using the Operetta High Content Imaging System PDF  454 KB
Case Study Inhibition of MicroRNA Sensitizes 3D Breast Cancer Microtissues to Radiation Therapy PDF  786 KB
Link For comprehensive information about our products, technologies and software for high-content screening and analysis, please visit our microsite Link
Technical Note Opera Phenix High-Content Screening System Technical Performance: Speed Link
Video Tutorials Harmony video tutorial series Link