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GTP Gamma S G-Protein Coupled SPA Assay Kit

SPA bead-based GTPγS binding (GPCR) assay kit.

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RPNQ0210
2748.00 EUR
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Detail Information

G-Protein Coupled Receptor Binding Assay contains the following components*:


  • WGA PVT SPA beads
  • 5 × assay buffer
  • Instructions for use

*In addition, the user will require guanosine
5'-[γ-35S] thiophosphate, triethylammonium salt,
([35S]GTPγS). This product is not supplied as part
of the kit.

Specifications

Detection Method Radiometric
Shipping Condition Dry Ice
Unit Size 1 kit
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Flyer

PerkinElmer Spa Reagents and Technologies

SPA reagents and CytoStar-T™ microplates let you see what you can’t see with any other technology, so your research becomes more productive. SPA from PerkinElmer. The advantages become even clearer at www.perkinelmer.com/spa.

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Manual

Manual for SPA G-Protein Coupled Receptor Assay

The SPA G-protein coupled receptor assay is a single well homogeneous technique using the scintillation proximity assay (SPA) principle. The SPA system is based on a polyvinyltoluene bead containing scintillant. The outer surface of the bead has been modified by a coating of wheat-germ agglutinin (WGA) which enables receptors to bind. The beads are allowed to interact with the receptor to enable binding to occur.

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Poster

Signal Transduction Assay for Arachidonic Acid Release from Chomo1 Cells Using Cytostar-T Scintillating Microplates

G-protein coupled receptors (GPCRs) possiblyrepresent the most important class of proteins fordrug discovery. Up to 40% of clinically marketeddrugs are active at this receptor family. However,these drugs exhibit their activity at less than 10% ofknown GPCRs. Thus, a major challenge for the drugdevelopment industry is to associate the manyorphan GPCRs with disease to potentially identifynovel pharmaceutical agents of the future.Receptors in this group act by facilitating the bindingof guanosine triphosphate (GTP) to specific Gproteins.The binding of GTP to the a subunit can beused to develop a functional assay for G-proteinactivation (GTP?S, see below) (1). GTP bindingactivates the G-protein, such that it, in turn

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