PerkinElmer
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Flexar UHPLC

For demanding applications when you need to collect more information in less time, our Flexar UHPLC is the perfect solution. This UHPLC is designed for a high productivity environment and delivers high sensitivity and resolution, exceptional flow accuracy and precision and faster results.

Part Number N2910535
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Detail Information

When you need the ultimate performance for your LC analysis, look no further than the Flexar UHPLC. This ultra- high performance UHPLC system delivers the highest resolution, highest sensitivity and fastest analysis. Across food, consumer product, pharmaceutical, industrial and environmental applications, the Flexar UHPLC performs reliably and with the flexibility to perform quality testing of raw materials, determine fraud/adulteration of products, ensure lot to lot consistency, as well research-based analysis for new products. Our Flexar UHPLC is engineered for labs that need more uptime, less maintenance and the ability to handle multiple methods – and its low carry over design enables quantitatively more accurate and repeatable results. Additional flexibility includes the choice of our powerfully easy-to-use Chromera® chromatography data system (CDS) software or TotalChrom® for for either single workstation or enterprise-wide control and regulatory compliance.

Our Flexar UHPLC is configured to provide the highest flow rate up to 5mL/min. with a maximum of 18K psi, a high speed autosampler for fast injection rates, choice of ovens and detectors, including the PDA Plus, which offers the highest acquisition rates in the industry.

Specifications

Model Name FX-20 Pump

Configurations

System Components
Description Part Number

Pump

FX-20 Pump

 

N2910535

Autosampler

UHPLC Autosampler

 

N2930664

Ovens

Standard Column Oven

Peltier Column Oven

 

N2601681

N2601680

Detectors

FX-UV/Vis Detector

PDA Plus Detector

 

N2920013

N2950010

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Application Note

A Robust Method for the Analysis of Commonly Used Sunscreen Compounds for Compliance with New FDA Regulations

Over time, exposure to ultra violet (UV) ,radiation from the sun or tanning beds can ,damage the skin’s cellular DNA, resulting in ,mutations that cause 3.5 million cases of skin ,cancers and about 11,500 deaths in the U.S. ,each year, for a total cost of nearly $2 billion. ,There are three types of UV: UVC, UVB, and ,UVA.

PDF 1 MB
Analysis of Biocides with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector

Biocides are chemical substances that are used to kill or inhibit harmful organisms. Biocides have a wide range of applications in consumer and industrial products.

PDF 955 KB
Analysis of Chlorophenols in Water by UHPLC with PDA Detection

This application will focus on the solid phase extraction (SPE) and HPLC analysis of chlorophenols in three tap water samples.

PDF 989 KB
Analysis of Common Antioxidants in Edible Oil with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector

Phenolic antioxidants and ascorbyl palmitate are commonly used in food to prevent the oxidation of oils. Oxidized oils cause foul odor and rancidity in food products.

PDF 1 MB
Analysis of Fat-Soluble Vitamins by UHPLC Using UV Detection

In 2009, the United States Pharmacopeial Convention introduced the USP Dietary Supplements Compendium (DSC) – an industry directed resource featuring regulatory guidance, documents, supplemental information, and reference tools.

PDF 1 MB
Analysis of Ginsenosides in Ginseng Root with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector

The root of the panax genus plant (also called Ginseng) has been used as an herbal medicine in Asia for over two thousand years for its purported various health benefits, including (but not limited to), antioxidant, anticarcinogenic, antiinflammatory, antihypertensive and anti-diabetic.

PDF 511 KB
Analysis of Isoflavones in Soy Products by UHPLC with UV Detection

The goal of this work was to develop a simpler, faster and reliable LC method for the analysis of the six most widely-used isoflavones in soy products.

PDF 1 MB
Analysis of Isoflavones with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector

Foods from plants are complex mixtures of chemicals including both essential nutrients and biologically active non-essential nutrients, referred to as phytochemicals.

PDF 992 KB
Analysis of Patulin in Apple Juice by UHPLC with UV Detection

The focus in this work was to develop a simple, robust, and reliable LC method for the analysis of patulin in apple juice.

PDF 1 MB
Analysis of Vanillin, Ethyl Vanillin, and Coumarin in Vanilla Extract Products by UHPLC with PDA Detection

With the focus on possible vanilla extract adulteration, this application focuses on the HPLC separation and quantitation of vanillin, ethyl vanillin, and coumarin in three store-bought vanilla extracts.

PDF 1 MB
Analysis of the Mycotoxin Patulin in Apple Juice Using the Flexar FX-15 UHPLC-UV

Patulin is produced by various molds, which primarily infect the moldy part of apples. Removing the moldy and damaged parts of the fruit may not eliminate all the patulin because some of it may migrate into sound parts of the flesh.

PDF 913 KB
Determination of a-acids in Hops and Beers

One essential aspect of the quality control in beer brewing is making sure that the type and

PDF 906 KB
Fast Analysis of Fat-Soluble Vitamins Using Flexar FX-10 and Chromera CDS

Sweeteners are low or zero-calorie sugar substitutes that are added in drinks, processed foods and pharmaceutical products to provide the sweet taste of table sugar, which is also called sucrose.

PDF 1 MB
PAHs in Surface Water by PDA and Fluorescence Detection Application Note

Heightened awareness of polycyclic aromatic hydrocarbons (PAHs) has become prevalent due to urban background levels found in surface water, soil, air, cosmetics and food. They are generated by the combustion of fossil fuels and are always found as a mixture of individual compounds that differ in behavior, environmental distribution, and their effect on biological systems. PAHs encompass a wide molecular weight range, differing based on their physical, chemical, and biological characteristics. PAHs in surface water result from a variety of sources including residential, industrial and commercial outlets, streets and parking lots, and atmospheric fallout. In this application, via a spiking experiment, we explore the levels at which PAHs in surface water can be monitored by UHPLC with a sub-2 µm particle sized column combined with photo diode array (PDA) and fluorescence (FL) detection.,

PDF 1 MB
Quantification of Benzodiazepines in Plasma Using UHPLC Time-of-flight Mass Spectrometry

Presented is an alternative technique to quantitate benzodiazepines in plasma using a rapid protein precipitation method with a fast LC separation method in combination with TOF-MS.

PDF 1 MB
Rapid UHPLC Determination of Common Preservatives in Cosmetic Products

This application note will present a fast, sensitive and reliable UHPLC analysis of six common parabens. A PerkinElmer Flexar FX-15 UHPLC system fitted with a Flexar FX PDA photodiode array detector was used. The separation was achieved using a PerkinElmer Brownlee Analytical C18, 1.9 µm 50 mm x 2.1 mm column.

PDF 960 KB
Simultaneous Analysis of Nine Food Additives with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector

This application note presents a fast and robust liquid chromatography method to simultaneously test nine widely used additives. Among the additives tested are: preservatives (benzoic acid, sorbic acid, dehydroacetic acid and methylparaben); artificial sweeteners (acesulfame potassium, saccharin and aspartame); flavoring agent (quinine); and a stimulant (caffeine). Method conditions and performance data including precision, accuracy and linearity are presented. The method is applied to a mouthwash and a tonic soda and the type and amount of additives are confirmed.

PDF 938 KB
The Analysis of Sunscreen-Active Ingredients and Parabens in Lotions and Lip Balms by UHPLC with PDA Detection

Individuals typically use 5-20 cosmetics per day, many of which contain sunscreen to prevent skin damage from the sun’s radiation, and antimicrobial preservatives called parabens. Although sunscreen-active ingredients are designed to block UV radiation, some cell damage may be caused when these ingredients are illuminated by sunlight after absorption into the skin. For example, oxybenzone, an ingredient considered safe by the FDA (Food and Drug Administration), is believed to contribute to the recent rise in melanoma cases by increasing the production of DNA-attacking free radicals upon UV exposure. Additionally, studies have shown oxybenzone to behave similarly to the hormone estrogen, suggesting that it may also contribute to the development of breast cancer. Parabens are absorbed through the skin via cosmetic applications and can be found in nearly all adult urine samples, with the highest concentrations observed in adult females and adolescents. Furthermore, parabens are thought to have estrogenic activity, which affects the expression of genes regulated by the natural form of estrogen, leading to early puberty in girls and an increased risk for the development of breast cancer.

PDF 890 KB
The Qualitative and Quantitative Analysis of a-Acids in Hops and Beers by UHPLC with UV Detection

Alpha acids (a-acids) are a class of chemical compounds of primary importance in the production of beer. They are found in the resin glands of the flowers of the hop plant (Humulus lupulus) and are normally added to the boil after mashing the grains, providing beers with their aroma and bitter taste. The a-acids found in hop resins are isomerized to form the iso-a-acids during prolonged boiling in the wort. The degree of isomerization and the amount of bitter taste produced by the addition of hops is highly dependent on the type of hop and the length of time the hops are boiled. Longer boil times will result in isomerization of more of the available a-acids, making the beer more bitter. The a-acid percentages vary within specific varieties of hops, depending on the growing conditions, drying methods, age of hops, climate and other factors. Figure 1 shows the common a-acids and iso-a-acids involved in the beer brewing process. Since the quality and quantity of a-acids is so important in consistently providing individual beers with their recognizable taste, it is essential to monitor their amount in hops and beers and to monitor the formation of the iso-a-acids during the beer brewing process. The focus of this application note is to provide an easy, straightforward, and robust analytical method for establishing the type and amount of a-acids in hops pellets, as well as determining the amount of a-acids and iso-a-acids in various beers.

PDF 2 MB

Brochure

QSight Triple Quadrupole LC/MS/MS

Whether you’re a lab manager or a bench chemist, you know firsthand how things are changing quickly in food and environmental analysis. And your lab needs to change to keep pace.

PDF 8 MB