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DELFIA® EuTDA Cytotoxicity Reagents

DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and ADCC.

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Detail Information

DELFIA® cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

Reagents for the assay are also available separately: 4005-0010 Lysis buffer (30 mL), C135-100 DELFIA Eu-Solution (200mL), C136-100 DELFIA BATDA labeling reagent (50µL).

Specifications

Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), DELFIA TRF
Experimental Type In vitro
Product Brand Name DELFIA
Quantity in a Package Amount 960.0 Units
Shipping Condition Blue Ice
Unit Size 1 Kit
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Application Note

A Simplified, Gentle Cell-Labelling Method for Non-Radioactive Cytotoxicity Assays

Discover PerkinElmer's simplified, gentle cell-labelling method for non-radioactive cytotoxicity assays

PDF 497 KB

Brochure

Drug Discovery Screening Solutions Brochure

Info on PerkinElmer's complete solutions for high-throughput and phenotypic screening.

PDF 880 KB

Manual

DELFIA Cell Cytotoxicity Manual

This product is intended for loading of cells to be used in short term cytotoxicity tests performed utilizing time-resolved fluorometry in the detection.

PDF 55 KB