PerkinElmer
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DELFIA Eu-Labeling kit (Eu-N1-ITC chelate), 0.2mg

DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.

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1244-302
476.00 EUR
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Detail Information

The DELFIA Eu-N1 ITC chelate is optimized for the europium labeling of proteins and peptides for use in dissociation-enhanced time-resolved fluorometric assays. Isothiocyanate (ITC) activated chelates react primarily with free amine groups such as those at the N-terminus of protein or lysine residues.

Each kit contains 0.2 mg labeling reagent plus Eu-standard, DELFIA Enhancement Solution, Eu-stabilizer, purified BSA for increasing the stability of labeled proteins, an uncoated microtitration strip plate, DELFIA Assay Buffer and DELFIA Wash Concentrate. The kit contents are sufficient for a labeling of up to 1 mg of protein or antibody.

Specifications

Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), DELFIA TRF
Experimental Type In vitro
Fluorophore Eu-N1-ITC
Product Brand Name DELFIA
Quantity in a Package Amount 0.2 mg
Shipping Condition Blue Ice
Unit Size 1 Kit
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Application Note

DELFIA® assays bring convenience in monoclonal anitbody development

The DELFIA® system provides a method of discovering specific, high-affinity monoclonal antibodies (Mabs) even in low concentrations in hybridoma supernatants. The assay used in these, conditions has to be very sensitive, specific, reliable, reproducible and easy enough to allow handling of about 1,000 to 1,500 samples in one batch.

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Manual

DELFIA Eu-Labelling Reagent 1244-301

DELFIA Eu-labeling Reagent is optimized for the europium labeling of proteins and peptides.

PDF 106 KB