PerkinElmer
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Cell Line Screening and Cherry Picking

This integrated robotic system consists of two integrated workstations; one for screening and one subsequent picking of antibody-producing hybridoma cells.

Part Number EG31009
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For research use only. Not for use in diagnostic procedures.

Detail Information

Configuration Requirements:

  • LIMS system to control all functions of each module/workstation with data exchange capabilities via XML files
  • Automation supporting high-throughput screening (>750 x 96 plates in 24 hours)
  • Automation to support cherry picking (>14,500 picks in 24 hours)
  • Liquid handling to accommodate cherry picking, optional assay plate preparation and exchange of cell culture medium
  • ‘Class 100’ clean room environment
  • 24/7 performance for complete ‘walk away’ automation

To maximize resources and increase productivity, biotechnology organizations often require their instruments to perform with a 24/7 operations capacity. In this example, PerkinElmer developed a workstation platform that could support both cell line screening and cherry picking application techniques in a standardized operating environment.

To address the throughput requirements of cell line screening, this explorer G3 integrated HCS and hybridoma screening workstation configuration included a unique turntable concept which allows multiple microplates to be handled in parallel. For high-throughput cherry picking applications it is essential to reduce plate exchange times to a minimum.

With the module designed for this application, the required reductions in plate exchange time were achieved by integrating a turntable in front of the incubator. With four plate buffer positions, a turntable can receive a picked plate and immediately provide a new “to be picked” plate, thereby reducing the time consuming import and export steps which can be further delayed when the liquid handling instrument is occupied with performing the ‘picking’. To transport the microplates from the turntable to the liquid handling instrument, we integrated a 6-axis industrial robot, which also served as the means for loading the microplates into the integrated barcode labeler. Moreover, each workstation module was outfitted with a 3x HEPA filter to create and ensure a ‘Class 100’ clean room environment.