Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
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Biotin labeled dideoxyguanosine triphosphate (ddUTP) is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. Biotin-labeled products may be detected with streptavidin conjugates. For the highest sensitivity in detection, we recommend using a streptavidin-HRP conjugate with tyramide signal amplification (TSA).
The number “11” in the compound name refers to the length of the linker arm between ddGTP and the label.
|Resource Type||File Name||File Format|
|Application Note||Signal Amplification Strategies for Rapid and Sensitive High Content Analysis||PDF 776 KB|
|Data Sheet||NEL547 - BIOTIN-11-ddUTP - Technical Data Sheet||PDF 55 KB|