PerkinElmer
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Biotin-11-ddCTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.

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NEL546001EA
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Detail Information

Biotin labeled dideoxycytosine triphosphate (ddCTP) is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. Biotin-labeled products may be detected with streptavidin conjugates. For the highest sensitivity in detection, we recommend using a streptavidin-HRP conjugate with tyramide signal amplification (TSA).

The number “11” in the compound name refers to the length of the linker arm between ddCTP and the label.

Specifications

Hapten Biotin
Nucleotide ddCTP
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Application Note

Signal Amplification Strategies for Rapid and Sensitive High Content Analysis

TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

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Data Sheet

NEL546 - BIOTIN-11-ddCTP - Technical Data Sheet

Nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerases. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used.

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