ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids.
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ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. Our ATPlite 3D and ATPlite 1step 3D luminescence assay systems use patented innovative technologies that measure cell proliferation and cytotoxicity in 3D cultured mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.
ATPlite™ 3D is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence assay is the alternative to colorimetric, fluorometric, high content microscopic analysis and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.
Storage: Buffer, MCLS and vials should be stored at 4°C. Microplates and TopSeal-A can be stored at Room Temperature.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Product Brand Name||ATPlite 3D|
|Unit Size||300 assay points (96-well)|
|Resource Type||File Name||File Format|
|Guide||Quick start guide for ATPlite 3D||PDF 639 KB|
|Application Note||Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer||PDF 1 MB|
|Application Note||Proliferation and Cell Death Analyses of 3D Cultures Using ATPlite 3D||PDF 1 MB|
|Flyer||Add a new dimension to your research||PDF 925 KB|