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Anti-Mouse IgG AlphaPlex-545 (Tb) Acceptor Beads, 25 mg

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-mouse IgG antibody, for use in Alpha no-wash multiplexing assays.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AP105TB-C
250 µg
610.00 EUR
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AP105TB-M
5 mg
6100.00 EUR
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AP105TB-R
25 mg
25000.00 EUR
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Detail Information

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-mouse IgG antibody. These beads are used to capture mouse IgG antibodies (targeting the Fc region), and can be used in conjunction with Alpha Donor beads to create no-wash assays for:

  • Analyte detection
  • Biomarker detection
  • Antibody detection
  • Antibody binding
  • Protein-protein interactions
  • Other immunoassays

AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay:

  • 250 µg of Acceptor beads is sufficient to run 250-500 wells using a 50 µL reaction volume
  • 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume
  • 5 mg of Acceptor beads is sufficient to run 5,000-10,000 wells using a 50 µL reaction volume

Specifications

Antibody Conjugates Anti-mouse IgG
Automation Compatible Yes
Bead Type or Core Bead Type AlphaPlex Terbium (545) Acceptor
Detection Method Alpha
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Unit Size 25 mg
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Brochure

Guide

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB