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3-Amino-3-Deoxydigoxigenin-9-ddGTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.

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NEL573001EA
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Detail Information

Aminodigoxigenin (DIG) labeled labeled dideoxyguanosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. Aminodigoxigenin-labeled products may be detected with anti-DIG antibody conjugates. For the highest sensitivity in detection, we recommend using an anti-DIG-HRP conjugate with tyramide signal amplification (TSA).

The number "9" in the compound name refers to the length of the linker arm between dCTP and the label.

Specifications

Hapten DIG
Nucleotide ddGTP
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Application Note

Signal Amplification Strategies for Rapid and Sensitive High Content Analysis

TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

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Data Sheet

NEL573 - 3-Amino-3-deoxydigoxigenin-9-ddGTP

2’,3’-dideoxynucleotide analogs are biologically active with a variety of DNA polymerases, and are chain terminators. Some analogs demonstrate variations in relative performance depending upon nucleotide and label (fluorophore or hapten) selected due to enzyme preferences.

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