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AlphaScreen General IgG (Protein A) Detection Kit, 10,000 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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6760617HV
100 assay points
93.00 EUR
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6760617C
500 assay points
187.00 EUR
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6760617M
10,000 assay points
2986.00 EUR
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6760617R
50,000 assay points
14300.00 EUR
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Detail Information

This bead kit can be used for many applications, including:

  • Antibody detection
  • Antibody binding
  • Binding assays
  • Enzymatic assays

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The AlphaScreen Protein A Acceptor beads are used to capture an antibody. The assay is set up to bring the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction.  This kit contains enough reagents to run 10,000 wells in 384-well format, using a 25 µL reaction volume.

Specifications

Antibody Conjugates Streptavidin/Protein A
Automation Compatible Yes
Product Brand Name AlphaScreen
Quantity in a Package Amount 10000.0 Units
Shipping Condition Blue Ice
Unit Size 10,000 assay points
Resources, Events & More
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Application Note

AlphaScreen™ MAP Kinase Assay

AlphaScreen™ MAP Kinase Assay was designed to measure ERK1/2 MAP kinase activity present in purified enzyme preparations or in cell extracts. The assay was developed using a very active ERK1 preparation. However, it can easily be applied to ERK1 extracted from other sources and to other MAP kinases.

PDF 605 KB

Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 4 MB

Poster

Development of a Homogeneous p38 Kinase Assay using AlphaScreen™ Technology

AlphaScreen™ is a homogeneous, luminescent proximity assay useful for studying a wide variety of biomolecular interactions. Here, we report the development of an AlphaScreen p38 kinase assay by monitoring the phosphorylation of activating transcription factor 2 (ATF-2).

PDF 275 KB