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Nickel Chelate Alpha Donor beads, 1 mg

Alpha Donor beads conjugated to nickel chelate. This bead can be used to capture His-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AS101D
1 mg
500.00 EUR
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AS101M
5 mg
1951.00 EUR
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AS101R
25 mg
9100.00 EUR
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Detail Information

Alpha Donor beads coated with nickel chelate. These beads can be used to capture His-tagged proteins and biomolecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

Specifications

Antibody Conjugates Nickel chelate
Automation Compatible Yes
Bead Type or Core Bead Type Alpha Donor
Detection Method Alpha
Experimental Type In vitro
Product Brand Name Alpha
Shipping Condition Blue Ice
Unit Size 1 mg
Resources, Events & More
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Brochure

Handle Large Biomolecular Interactions With Ease

The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measurement of protein interactions and complexes as large as 200 nm in size

PDF 798 KB

Data Sheet

AlphaScreen Nickel Chelate Donor beads

AlphaScreen Nickel Chelate Donor Beads at 5 mg/mL in 25 mM Hepes, pH 7.4, 100 mMNaCl supplemented with 0.05% Proclin-300 as a preservative.

PDF 114 KB

Guide

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB