PerkinElmer
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Unconjugated AlphaPlex-545 Acceptor Beads, 50 mg

Unconjugated AlphaPlex 545 (Terbium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
6782001
1 mg
1393.00 EUR
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6782002
5 mg
4880.00 EUR
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6782003
50 mg
29300.00 EUR
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Detail Information

AlphaPlex is a multiplexing no-wash ELISA alternative and can be used for applications such as:

  • Analyte detection assays
  • Protein-protein interaction assays
  • Protein-DNA interaction assays
  • Protein-RNA interaction assays
  • Protein-small molecule interaction assays
  • Protein detection assays
  • Antibody detection assays
  • Antibody binding assays
  • Immunogenicity assays
  • Enzymatic assays

AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume.

Specifications

Antibody Conjugates Unconjugated
Automation Compatible Yes
Bead Type or Core Bead Type AlphaPlex Terbium (545) Acceptor
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaPlex
Quantity in a Package Amount 1.0 Units
Shipping Condition Blue Ice
Unit Size 50 mg
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Brochure

Guide

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB