PerkinElmer
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AlphaLISA universal buffer, 5X

Pre-formulated buffer used to QC Alpha assays. This buffer can sometimes be used for protein-protein binding, proof-of-concept, and other Alpha assays.

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For research use only. Not for use in diagnostic procedures.

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Unit Size
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AL001C
10 mL
209.00 EUR
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AL001F
100 mL
399.00 EUR
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Detail Information

Alpha no-wash assay technology (AlphaLISA and AlphaScreen assays) is widely used in both basic research and in high throughput screening. Given the wide range of possible applications for Alpha assays, many different buffer types and compositions may be considered for use in Alpha assays. We provide a selection of buffers validated for AlphaLISA assays that may be ideal for your needs.

Specifications

Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Unit Size 10 mL
Resources, Events & More
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Brochure

Poster

All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures

Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Integral plasma membrane (EGFR) and secreted solubleproteins (TNFa, IL1ß, IL6, IL8) were successfully determined, on adherent or suspension cells, using the standard AlphaLISA Immunoassay buffer.

PDF 288 KB
Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines

Enzyme-linked Immunosorbent Assay is the most widely Kits adopted method for detection and quantification of cytokines and other biomarkers. This traditional technology offers good,selectivity, sensitivity and assay versatility; however, it has certain disadvantages such as limited dynamic range and low throughput due to the numerous wash steps.

PDF 279 KB