Pre-formulated buffer used to QC Alpha assays. This buffer can sometimes be used for protein-protein binding, proof-of-concept, and other Alpha assays.
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For research use only. Not for use in diagnostic procedures.
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Alpha no-wash assay technology (AlphaLISA and AlphaScreen assays) is widely used in both basic research and in high throughput screening. Given the wide range of possible applications for Alpha assays, many different buffer types and compositions may be considered for use in Alpha assays. We provide a selection of buffers validated for AlphaLISA assays that may be ideal for your needs.
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||10 mL|
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Integral plasma membrane (EGFR) and secreted solubleproteins (TNFa, IL1ß, IL6, IL8) were successfully determined, on adherent or suspension cells, using the standard AlphaLISA Immunoassay buffer.
Enzyme-linked Immunosorbent Assay is the most widely Kits adopted method for detection and quantification of cytokines and other biomarkers. This traditional technology offers good,selectivity, sensitivity and assay versatility; however, it has certain disadvantages such as limited dynamic range and low throughput due to the numerous wash steps.