PerkinElmer
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Myeloperoxidase (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Myeloperoxidase (MPO) Detection Kit is designed for detection and quantitation of human MPO in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AL245C
500 assay points
3228.00 EUR
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AL245F
5,000 assay points
23600.00 EUR
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Detail Information

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Myeloperoxidase (MPO) is a glycosylated and heme-containing enzyme of approximately 80 kDa. The enzyme is stored in azurophilic granules of polymorphonuclear neutrophils and macrophages, and is released into extracellular fluid in the setting of inflammatory processes. MPO participates in the innate immune defense mechanism through formation of microbicidal reactive oxidants and diffusible radical species. Its involvement in oxidative stress and inflammation makes it a possible marker for atherosclerotic plaque instability and a clinical tool for the evaluation of coronary heart disease.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target Myeloperoxidase
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cardiovascular
Unit Size 5,000 assay points