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CCL2/MCP1 (mouse/rat) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Mouse/Rat C-C Motif Chemokine 2/Monocyte Chemoattractant Protein 1 (CCL2/MCP1) Detection Kit is designed for detection and quantitation of mouse or rat CCL2/MCP1 in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Mouse CCL2/MCP1 can be quantitatively measured in mouse serum. The analyte in this kit is mouse CCL2/MCP1.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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AL509C
500 assay points
3023.00 EUR
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AL509F
5,000 assay points
23600.00 EUR
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Detail Information

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Mouse and rat C-C Motif Chemokine 2 (CCL2) or Monocyte Chemoattractant Protein 1 (MCP1) contain 125 amino acids and are glycosylated. CCL2 is part of the CXC subfamily of cytokines. In mouse, fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can produce CCL2 either constitutively or upon stimulation. CCL2 displays chemotactic activity for monocytes and basophils, but not for neutrophils or eosinophils. It also regulates adhesion molecule expression and cytokine production in mouse monocytes. CCL2 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. CCL2 is considered as an important biomarker in cardiovascular diseases.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target CCL2
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Mouse
Therapeutic Area Inflammation
Unit Size 5,000 assay points
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Brochure

Poster

AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices

The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.

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