PerkinElmer
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IL-4 (bovine) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for bovine IL-4 enables the detection and quantitation of bovine interleukin-4 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AL542HV
100 assay points
462.00 EUR
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AL542C
500 assay points
1532.00 EUR
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AL542F
5,000 assay points
10100.00 EUR
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Cytokines are soluble mediators that impact a multitude of biologies including cell proliferation, survival, death, motility, cell-cell and cell-matrix interactions as well as immune response and leukocyte infiltration. Cytokines are intimately associated with acute and chronic diseases and response to vaccination. Interleukin 4 (IL4) is a cytokine of many biological roles, including stimulation of activated B-cell and T-cell proliferation, differentiation of naive helper T cells (Th0 cells) into Th2 cells, and of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE and up-regulates MHC class II production. Overproduction of IL4 is associated with allergies. IL4, along with other Th2 cytokines, is involved in the airway inflammation with allergic asthma.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target IL4
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Bovine
Therapeutic Area Inflammation
Unit Size 500 assay points