check quantity

IL-2 (bovine) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for bovine IL-2 enables the detection and quantitation of bovine interleukin-2 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

Please enter valid quantity

Please login to add favorites


Part Number Unit Size List Price Your Price Quantity
AL543HV 100 assay points 462.00 EUR
AL543C 500 assay points 1532.00 EUR
AL543F 5,000 assay points 10100.00 EUR
Buy Now

You successfully added item(s) to your cart



  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Cytokines are soluble mediators that impact a multitude of biologies including cell proliferation, survival, death, motility, cell-cell and cell-matrix interactions as well as immune response and leukocyte infiltration. Cytokines are intimately associated with acute and chronic diseases and response to vaccination. IL2 plays key roles in tolerance and immunity, primarily via its direct effects on T cells. Bovine Interleukin 2 (bIL2) is a 15 kDa cytokine produced primarily by mitogen- or antigen-activated T lymphocytes. It plays a key role in promoting clonal expansion of antigen-specific T cells. It can stimulate the growth and expansion of a multitude of cells as well as induce cytokine production and cytolytic activity by natural killer cells.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.


Assay Target IL2
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Bovine
Therapeutic Area Inflammation
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  128 KB
Application Note Antigen-Stimulated PBMC Cytokine Release Measured by AlphaLISA Bovine Cytokine Kits PDF  3 MB
Manual AlphaLISA bovine IL-2 Kit AL543 PDF  611 KB
Event Society of Laboratory Automation & Screening (SLAS)