The AlphaLISA® Human Interleukin 18 (IL-18) Detection Kit is designed for detection and quantitation of human IL-18 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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For research use only. Not for use in diagnostic procedures.
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Interleukin 18 (IL18) is a 18 kDa cytokine produced by Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, and adrenal cortex cells. IL18 is involved in the production of interferon-? (IFN-?) in response to toxic shock and shares functional similarities with IL12. IL18 works together with IL12 to induce cell-mediated immunity following infection with microbial products like lipopolysaccharides (LPS). IL18 is also able to induce severe inflammatory reactions, which suggests a role in certain inflammatory disorders. Elevated plasma levels of IL18 have been reported in hematological malignancies, inflammatory diseases, and autoimmune disorders.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Assay Target | IL18 |
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Assay Target Class | Cytokine |
Automation Compatible | Yes |
Detection Method | Alpha |
Experimental Type | In vitro |
Product Brand Name | AlphaLISA |
Shipping Condition | Blue Ice |
Target Species | Human |
Therapeutic Area | Inflammation |
Unit Size | 500 assay points |
Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Integral plasma membrane (EGFR) and secreted solubleproteins (TNFa, IL1ß, IL6, IL8) were successfully determined, on adherent or suspension cells, using the standard AlphaLISA Immunoassay buffer.
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.
Enzyme-linked Immunosorbent Assay is the most widely Kits adopted method for detection and quantification of cytokines and other biomarkers. This traditional technology offers good,selectivity, sensitivity and assay versatility; however, it has certain disadvantages such as limited dynamic range and low throughput due to the numerous wash steps.