PerkinElmer
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IL-8 (porcine) AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® immunoassay kit for porcine interleukin 8 (IL-8) enables the quantitative determination of porcine IL-8 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL571HV 100 Assay Points 1104.00 EUR
AL571C 500 Assay Points 2139.00 EUR
AL571F 5,000 Assay Points 10700.00 EUR
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Interleukin-8 (IL-8), also known as CXCL8, is a chemokine produced by monocytes/macrophages, epithelial cells, endothelial cells and neutrophils, in response to inflammatory stimuli. IL8 is a potent chemoattractant for neutrophils, basophils, and T-cells, and causes degranulation of neutrophil specific granules as well as azurophilic granules. In addition, IL8 also has a wide range of other proinflammatory effects. IL8 has been linked to various pathologies, especially respiratory diseases such as pneumonia in porcine.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL8
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Porcine
Unit Size 5,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual Protocol for AlphaLISA porcine IL-8 detection assay PDF  456 KB