PerkinElmer
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E-Cadherin (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human E-cadherin enables the detection and quantitation of human E-cadherin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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AL370HV
100 assay points
462.00 EUR
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AL370C
500 assay points
1532.00 EUR
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AL370F
5,000 assay points
10100.00 EUR
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Epithelial Cadherin (E-Cadherin), also known as Cadherin-1 or Uvomorulin (in mouse and rat) is a single-pass transmembrane protein that facilitates calcium dependent cell adhesion. A member of the Cadherin family, E-Cadherin utilizes five extracellular EC domains to form cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. Cleavage of the N-terminal domain by a number of proteases is critical for cell motility and EGFR-dependent survival. The intracellular domain of E-cadherin interacts with many proteins including β-catenin, α-catenin, vinculin, and plakoglobin. Lack of binding to any of these proteins has been indicated in cancer metastasis.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target E-Cadherin
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 100 assay points