The AlphaLISA® immunoassay kit for human MMP-12 enables the detection and quantitation of human matrix metalloproteinase-12 in serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). The kit was designed to detect both MMP12 and pro-MMP12.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL375HV||100 assay points||462.00 EUR|
|AL375C||500 assay points||1532.00 EUR|
|AL375F||5,000 assay points||10100.00 EUR|
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Matrix metalloproteinase 12 (MMP12, macrophage elastase ), a member of the matrix metalloproteinase family, is an enzyme composed of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. MMP12 can degrade soluble and insoluble elastin and type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. Like other MMPs, MMP12 is involved in the degradation of basement membranes and thus cancer development. This kit has been designed for the detection of Human MMP12 in serum and cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 124 KB|
|Application Note||Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer||PDF 1 MB|
|Application Note||Detection and Modulation of Extracellular Matrix Protein Deregulation in Human Prostate Carcinoma||PDF 1 MB|
|Data Sheet||AlphaLISA Human MMP-12 Detection Kit AL375||PDF 457 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)|