PerkinElmer
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ApoB (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human ApoB enables the quantitative determination of human apolipoprotein B in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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AL392HV
100 Assay Points
623.00 EUR
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AL392C
500 Assay Points
1868.00 EUR
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AL392F
5,000 Assay Points
18700.00 EUR
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Apolipoprotein B (ApoB) is a major protein component of Low, Very low, and Intermediate Density Lipoproteins. Although the exact function of ApoB remains unclear it is apparent that ApoB plays a major role in organization and formation of these lipoprotein complexes. ApoB is currently used as a biomarker to predict cardiovascular diseases, where higher levels have been associated with an increased risk. This AlphaLISA detection kit allows for the detection of ApoB levels in serum, plasma, and cell culture supernatants.

Specifications

Assay Target ApoB
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 500 Assay Points
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Brochure

Manual

AlphaLISA Human ApoB Detection kit AL392

This manual explains how to run the AlphaLISA no-wash human ApoB detection assay.

PDF 418 KB