PerkinElmer
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Cry2A AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® Cry2A Detection Kit is designed for detection and quantitation of Cry2A in GMO seeds, leaves, and plant extracts in a homogeneous (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
AL403HV
100 assay points
622.00 EUR
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AL403C
500 assay points
1524.00 EUR
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AL403F
5,000 assay points
9700.00 EUR
more
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Detail Information

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Cry2A is an insecticidal protein and is produced by the naturally occurring soil bacterium Bacillus thuringiensis subsp. kurstaki (Btk). The protein is used extensively in agricultural farming as a direct application pesticide. The gene encoding Cry2A has been widely introduced to genomes of many crops (e.g. corn, cotton, and soybean) to create genetically modified organisms (GMO). During growth, genetically modified crops produce the Cry2A protein that confers protection against certain insect pests.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target Cry2A
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Quantity in a Package Amount 1.0 Units
Shipping Condition Blue Ice
Target Species Plant
Therapeutic Area Agriculture/Plant
Unit Size 100 assay points