The AlphaLISA® immunoassay kit for human CD86 enables the quantitative determination of human CD86 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL3045HV||100 Assay Points||623.00 EUR|
|AL3045C||500 Assay Points||1868.00 EUR|
|AL3045F||5,000 Assay Points||14900.00 EUR|
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells (APC) that provides costimulatory signals necessary for T cell activation and survival. It is the ligand for two proteins at the cell surface of T cells, CD28 and CTLA4 (cytotoxic T-lymphocyte-associated protein 4). Binding of this protein with CD28 is a costimulatory signal for activation of the T-cell. CD86 works in tandem with CD80 to prime T cells. Recent studies have revealed that CD86 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the CD86 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 128 KB|
|Application Note||Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction||PDF 3 MB|
|Application Note||Protocol Optimization for Detection and Quantification of Membrane-Bound Proteins Using AlphaLISA Te||PDF 2 MB|
|Application Note||Quantifying Changes in CD28 and CTLA-4 Levels in Peripheral Blood Mononuclear Cells with AlphaLISA||PDF 1 MB|
|Application Note||Utilizing AlphaLISA Technology to Screen for Inhibitors of the CTLA-4 Immune Checkpoint||PDF 1 MB|
|Manual||Protocol for AlphaLISA human CD86 Detection Kit||PDF 430 KB|
|Technical Note||AlphaLISA Assays for Immune Checkpoint Detection and Quantitation||PDF 532 KB|