Beta-2 Microglobulin (human) AlphaLISA Detection Kit, 500 assay points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Beta2-microglobulin is a small (99 amino acids) protein present as the light chain of the major immunocompatibility complex receptors and also the light chain of the CD1 receptor complex. The protein plays a critical role in both receptor maturation and in antigen expression at the surface of immune cells. It is essential for HLA recognition and binding of peptides. Beta2-microglobulin is also involved as a partner of the human hemochromatosis protein and as such helps regulate iron levels.

Elevated levels of the protein in serum are a marker of leukemia and multiple myeloma. Elevated levels in urine indicate kidney damage, while elevated levels in CSF indicate meningitis or brain tumors.