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AlphaLISA Anti-methyl Histone H3 Arginine 2 (H3R2me) Acceptor Beads, 250 µg

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is methylated at arginine 2 (H3R2me). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.

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For research use only. Not for use in diagnostic procedures.

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AL139C
250 µg
1249.00 EUR
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AL139M
5 mg
6500.00 EUR
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AL139R
25 mg
22700.00 EUR
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Detail Information

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is methylated at arginine 2(H3R2me) in a homogeneous AlphaLISA assay.

In the AlphaLISA assay, a biotinylated Histone H3 peptide substrate is used in a biochemical enzymatic reaction with histone methyltransferase (HMT) and S-adenosylmethionine (SAM) cofactor. When the enzymatic reaction is stopped, the level of methylation is determined using antibody-coated AlphaLISA Acceptor beads and streptavidin-coated Alpha Donor beads. The streptavidin Donor beads capture the biotin moeity on the peptide substrate, while the antibody on the AlphaLISA Acceptor beads recognizes the specific epigenetic mark (methylation of Histone H3 on arginine 2). Alternatively, a biotinylated Histone H3 (C-term) antibody (Cat. No. AL118) can be used to capture full-length Histone H3 substrates to the streptavidin Donor beads. Upon laser irradiation of the bead complexes at 680 nm, short-lived singlet oxygen molecules produced by the Donor beads can reach the Acceptor beads in proximity to generate an amplified chemiluminescent signal at 615 nm. The intensity of light emission is proportional to the level of substrate modification.

Features:

  • No-wash epigenetic assay
  • Fully-validated mark specificity
  • Substrate flexibility (peptide, protein, histone, nucleosome substrates)
  • Easy-to-automate
  • Fast assay optimization

Specifications

Antibody Conjugates Anti-H3R2me
Automation Compatible Yes
Bead Type or Core Bead Type AlphaLISA Acceptor
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Methylation
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Unit Size 250 µg
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Brochure

EPIGENETICS DRUG DISCOVERY SOLUTIONS

Epigenetics, the study of changes in gene expression, is a rapidly growing field which, presents a significant opportunity for drug discovery. As the understanding of the role, of epigenetic changes in various disease states advances, the need for high quality, reagents that can rapidly identify and optimize therapeutic molecules that target these, enzymes is increasing.

PDF 666 KB

Technical Note

AlphaLISA PRMT6 Histone H3-Arginine N-methyltransferase Assay

AlphaLISA technology is a powerful and versatile platform that offers, highly sensitive, no-wash immunoassays using Alpha Donor and, AlphaLISA Acceptor beads. In this technical note, we present the, optimization of a PRMT6 enzymatic assay using a biotinylated histone, H3-derived peptide as substrate. Detection of the methylated product, was performed by the addition of Streptavidin (SA) Alpha Donor, beads and AlphaLISA Acceptor beads conjugated to an antibody (Ab), directed against the methylated H3R2 residue. Upon laser irradiation, of the beads-target complexes at 680 nm, short-lived singlet oxygen, molecules produced by the Donor beads can reach the Acceptor, beads in proximity to generate an amplified chemiluminescent signal, at 615 nm. The intensity of the light emission is proportional to the, methylation activity of the PRMT6 enzyme.

PDF 853 KB