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Amyloid β 1-42 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for detection and quantitation of human Amyloid β 1-40 (Aβ 1-40) in cerebrospinal fluid (CSF), cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

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For research use only. Not for use in diagnostic procedures.

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Unit Size
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AL203C
500 assay points
3023.00 EUR
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AL203F
5,000 assay points
23600.00 EUR
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Detail Information

One antibody is specific to amino acids 17-24 (epitope VFFAE): mouse monoclonal antibody, clone number 4G8. The second antibody is specific to the C-terminus: mouse monoclonal antibody, clone number 12F4.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Amyloid beta (Aß) is a short peptide derived from the proteolysis of a larger transmembrane molecule, the amyloid precursor protein (APP). The ß- and γ-secretases cleave the respective N- and C-terminal ends of the Aß sequence, liberating the Aβ peptide from APP. Aß40 is the major species of Aß produced by neurons and other cells, and accounts for over 70% of total Aß produced, while the remaining 10-20% is comprised of the longer Aß42, and other species. Aß42 has a greater propensity to form aggregates or fibrils and also has a greater neuronal toxicity in tissue culture models than Aß40, implying that Aß42 is a more important factor in Alzheimer's disease (AD) pathogenesis and plaque formation. Levels of Aß42 in cerebrospinal fluid are decreased in the majority of AD subjects (probably due to its aggregation into plaques), making it an important biomarker for this disease.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target Aβ42
Assay Target Class Peptide
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Central Nervous System
Unit Size 5,000 assay points
Resources, Events & More
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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 4 MB

Poster

All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures

Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Integral plasma membrane (EGFR) and secreted solubleproteins (TNFa, IL1ß, IL6, IL8) were successfully determined, on adherent or suspension cells, using the standard AlphaLISA Immunoassay buffer.

PDF 288 KB
Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines

Enzyme-linked Immunosorbent Assay is the most widely Kits adopted method for detection and quantification of cytokines and other biomarkers. This traditional technology offers good,selectivity, sensitivity and assay versatility; however, it has certain disadvantages such as limited dynamic range and low throughput due to the numerous wash steps.

PDF 279 KB