PerkinElmer
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AlphaLISA 5X Epigenetics Buffer 1, 100 mL Kit

Pre-formulated buffer that can be used in histone H3 peptide-based epigenetic assays.

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For research use only. Not for use in diagnostic procedures.

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AL008C
10 mL
112.00 EUR
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AL008F
100 mL
606.00 EUR
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Detail Information

Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. This buffer prevents the non-specific binding of the highly-charged histone H3 (1-21) peptide to Donor and Acceptor beads in AlphaLISA epigenetic assays. Validated with Acceptor beads anti-H3K9ac (AL114), anti-H3K4me1-2 (AL116) and anti-H3K9me2 (AL117). It is used at the detection step for the dilution of Acceptor and Donor beads, not for the enzymatic reaction step. This buffer is not recommended for assays using full length histone proteins or nucleosomes.

Formulation: proprietary

Specifications

Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Unit Size 100 mL
Resources, Events & More
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Brochure

Handle Large Biomolecular Interactions With Ease

The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measurement of protein interactions and complexes as large as 200 nm in size

PDF 798 KB

Poster

AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices

The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.

PDF 273 KB
Development of Homogeneous Non-radioactive Assays for Studying Histone H3 Methyltransferases and Demethylases

Anti-mark antibodies coupled to AlphaLISA Acceptor beads or labeled with LANCE Ultra europium chelate were used for the successful optimization of robust and, sensitive epigenetic assays using histone H3-derived peptides as substrates.

PDF 381 KB
Development of Homogeneous Proximity Assays for JMJD2A/2C Histone Demethylases

In eukaryotes, the covalent modification of histones has a crucial role in chromatin architecture and plays an important part in a plethora of cellular processes, from chromatinre modeling and transcriptional regulation, to DNA repair and cell cycle control.

PDF 289 KB
Development of a Non-Radioactive, No-Wash Biochemical Assay for High-Throughput Screening of Small Molecule Modulators of DNA MethyltransferasesNathalie

Covalen modification of DNA through methylation is catalyzed by specific DNA methyltransferases (DNMTs).

PDF 386 KB

Technical Note

AlphaLISA SET7/9 Histone H3-Lysine N-methyltransferase Assay

The AlphaLISA technology allows performing no-wash homogeneous proximity immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of anepigenetic enzymatic assay using a biotinylated histone H3-derived peptide as substrate.

PDF 662 KB
AlphaLISA p300 Histone H3-Lysine Acetyltransferase Assay

The AlphaLISA technology allows performing no-wash homogeneous proximity immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of anepigenetic enzymatic assay using a biotinylated histone H3-derived peptide as substrate.

PDF 673 KB