check quantity

AlphaScreen SureFire Akt 1/2/3 Total Assay Kit - 10,000 Points

The AlphaScreen® SureFire® Akt 1/2/3 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1/2/3 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Please enter valid quantity

Please login to add favorites


Part Number Unit Size List Price Your Price Quantity
TGRTAPSHV100 100 assay points 424.00 EUR
TGRTAPS500 500 assay points 1549.00 EUR
TGRTAPS10K 10,000 assay points 6800.00 EUR
TGRTAPS50K 50,000 assay points 16700.00 EUR
Buy Now

You successfully added item(s) to your cart


Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening


  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.


Assay Pathway PI3K/Akt/mTOR
Assay Target Akt1/2/3
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaScreen SureFire
Quantity in a Package Amount 10000.0 Units
Shipping Condition Blue Ice
Target Species Human
Unit Size 10,000 assay points