The AlphaLISA® SureFire® Ultra™ p-TrkA (Tyr674/675)/p-Trk-B (Tyr706/707) assay is a sandwich immunoassay for quantitative detection of phospho-TrkA (phosphorylated on Tyr674/675) and/or pTrkB (phosphorylated on residues Tyr706/707) in cellular lysates using Alpha Technology.
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For research use only. Not for use in diagnostic procedures.
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In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA SureFire Ultra™ kits are compatible with:
Alpha SureFire kits can be used for:
|Assay Pathway||PI3K/Akt/mTOR, MAPK|
|Assay Target||TrkA, TrkB|
|Assay Target Class||Phosphoprotein|
|Product Brand Name||AlphaLISA SureFire Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|