Extra control lysate for the AlphaLISA SureFire Ultra phospho-mTOR assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-mTOR assay kit, but can also be ordered separately.
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For research use only. Not for use in diagnostic procedures.
This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-mTOR assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Jurkat cells were cultured in a T175 flask to approximately 1X106 cells/mL in 10% FBS containing medium, then treated with 2 μg/mL EGF for 30min and lysed at 1X107 cells/mL with 1X SureFire Ultra Lysis buffer.
Assay Pathway | PI3K/Akt/mTOR |
---|---|
Assay Target | mTOR |
Assay Target Class | Phosphoprotein |
Automation Compatible | Yes |
Detection Method | Alpha |
Product Brand Name | AlphaLISA SureFire Ultra |
Shipping Condition | Blue Ice |
Unit Size | Vial |