Extra control lysate for the AlphaLISA SureFire Ultra phospho-mTOR assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-mTOR assay kit, but can also be ordered separately.
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For research use only. Not for use in diagnostic procedures.
This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-mTOR assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Jurkat cells were cultured in a T175 flask to approximately 1X106 cells/mL in 10% FBS containing medium, then treated with 2 μg/mL EGF for 30min and lysed at 1X107 cells/mL with 1X SureFire Ultra Lysis buffer.
| Assay Pathway | PI3K/Akt/mTOR |
|---|---|
| Assay Target | mTOR |
| Assay Target Class | Phosphoprotein |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Product Brand Name | AlphaLISA SureFire Ultra |
| Shipping Condition | Blue Ice |
| Unit Size | Vial |
