PerkinElmer
check quantity

AlphaLISA SureFire Ultra Total GAPDH Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ Total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for cell number.

You successfully added item(s) to your cart

Part Number
Unit Size
List Price
Your Price
Quantity
ALSU-TGAPD-A-HV
100 Assay Points
508.00 EUR
more
ALSU-TGAPD-A500
500 Assay Points
1716.00 EUR
more
ALSU-TGAPD-A10K
10,000 Assay Points
9500.00 EUR
more
ALSU-TGAPD-A50K
50,000 Assay Points
29400.00 EUR
more
Buy Now

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Detail Information

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA® SureFire® Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

AlphaLISA® SureFire® Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Assay Target GAPDH
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA SureFire Ultra
Quantity in a Package Amount 100.0 Units
Shipping Condition Blue Ice
Target Species Human
Unit Size 100 Assay Points
Resources, Events & More
  • All

Brochure

Flyer

Manual

AlphaLISA SureFire GAPDH Detection Kit (HV - High Volume)

This manual explains how to run an AlphaLISA SureFire Ultra Assay for GAPDH (High volume kit)

PDF 516 KB