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Phospho-AMPKα 1/2 (Thr172) AlphaLISA SureFire Ultra Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra p-AMPKα 1/2 (Thr172) assay is a sandwich immunoassay for quantitative detection of phospho-AMPKα (phosphorylated on Thr172) in cellular lysates using Alpha Technology.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
ALSU-PAMPK-A-HV
100 Assay Points
508.00 EUR
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ALSU-PAMPK-A500
500 Assay Points
1716.00 EUR
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ALSU-PAMPK-A10K
10,000 Assay Points
9500.00 EUR
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ALSU-PAMPK-A50K
50,000 Assay Points
30600.00 EUR
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Detail Information

In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

  • The HV kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500 point kit contains reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000 point kit contains reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Assay Target AMPKα
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA SureFire Ultra
Shipping Condition Blue Ice
Unit Size 500 Assay Points